Some Applications for Cannabis Test Kit Use
For Medical Users
Test the strain yourself before you buy or ask your local cannabis provider to show you the menu according to analysis results. It is best to keep a journal of strains that you consume to treat your symptoms. As you find strains that work for you, you will see a correlation between the analysis and those particular strains.
For Pharmacist & Care-takers
Monitoring the percentage rate of change of the cannabinoids during storage, in terms of the amount of acids present and the presence of the degradation products (due to aging & oxidation) of THC (= CBN) and the forming of degradation products of CBD (= Canabielsoin) and CBC (= Cannabicyclol). Display the fingerprints and THC% of your varieties ‘'on the menu''
Clear information for the customers, on the desk!
Cannabis Testing is used for harvesting, storage and quality control.
For Horticulturists & Breeders
Monitoring the percentage rate of change of the cannabinoids in the profile & change in THC percentage by changing one of the environmental parameters during the growth stage.
Determine the difference in the cannabinoid profiles of your F1-offspring and compare the profiles with the parents, P1en P2 and make your selection!. It helps selecting appropriate strains to meet ‘'your'' needs
Variety recognition; The fingerprint reveals dominant, cannabinol expressing
genes in the offspring, from one or both of the parents P1 and P2.
The influences of maturity1, age, storage conditions and geographical origin on the cannabinoid profile of Cannabis species.
The biosynthesis of cannabinoids and other terpenes parallels the developmental stages of the calyx (five-part carpel structure of the staminate and pistillate flower.) and associated resin-producing glandular trichomes.
Determination of maturational stage and timing of the harvest is based on the average calyx and
resin condition (fingerprint) CAT is used to compare cannabinoid readouts to select parents for your breeding programme. If you were interested in breeding two strains, you would take fresh cannabis flowers from each strain and analyze them for cannabinoids. You can breed two strains that are complimentary or dissimilar ( for example both P1 are high in CBG ( = ‘'hashish'' like variety, and rare in the marihuana community), or one is high in CBG, or one is high in CBG and the other is high in CBN. Once you have bred the two, you can analyze the flowers of the offspring to see the results of your breeding programme, and observe hybrid vigour you've created, as well as her successive generations (FI, F2, F3).
Method of Testing
Thin-layer chromatography (TLC) is a chromatographic technique that in many (phyto-) pharmaceutical and analytical research laboratories is used for separation and identification by colouring (chroma =Greek for ‘'color'') of organic compounds in a mixture of hundreds of compounds, like our cannabinoid drop in figure 1.
TLC is a simple, quick, and inexpensive procedure that gives a quick answer as to how many components are in a mixture. TLC is also used to support the identity of a compound in a mixture when the Rf of a compound is compared with the Rf from the literature (see table 1). In our case, the cannabinoids can be identified by their specific color and specific location (Rf value) and order of appearance on the TLC plate, if carried out with the solvent system from the Kit.
A TLC plate is a sheet of glass which is coated with a thin layer of a solid adsorbent, silica. A small amount of the mixture to be analysed is spotted near the bottom of this plate. The TLC plate is then placed in a shallow pool of a solvent in a developing chamber so that only the very bottom of the plate is in the liquid. This liquid, or eluent or carrier fluid, is the mobile phase, and it slowly rises up the TLC plate by capillary action.
As the solvent moves, it will pass the little spot of cannabinoid-oil micro-droplet of 2 ul that was applied on the bottom of the plate. An equilibrium is established for each component of the mixture between the molecules of that component which are absorbed on the solid and the molecules which are in solution. In principle, the components will differ in solubility and in strength of their adsorption to the adsorbent and the other components will be carried farther up to the plate than the others. When the solvent has reached the top of the plate, the plate is removed from the developing chamber, dried (ventilation!) for 3 minutes. After drying, the separated components (cannabinoids) now separated in the silica plates, will be visualized by spraying the plate with a dye. The dye is highly specific for cannabinoids. Every principal cannabinoid will display his own color! THC: wine red, CBD: bright yellow, CBG: orange, CBN: violet etc..
Another way is UV detection of the spots under a UV lamp: the silica on the thin layer plates used in the Kit contains a fluor, this is a component that absorbs the uv wavelength of 254 nm and which fluoresces every where except where a cannabinoïd or other organic compound is on the plate.